Fast & Effective Removal of Interfering Proteins

Fast & Effective Removal of Interfering Proteins


  • Post By : Kumar Jeetendra

  • Source: Microbioz India

  • Date: 18 Mar,2020

Incorporating a novel dual hydrophobically treated matrix filter, Microlute™ Protein Precipitation Plate from Porvair Sciences set a new standard for labs looking to remove interfering proteins from serum, plasma or even whole blood samples prior to analysis by Liquid Chromatography / Mass Spectrometry (LC/MS).

Biological samples commonly contain proteins that interfere with downstream applications such as LC/MS analysis. Microlute™ Protein Precipitation Plates (PPP) use the CRASH method, in which protein is denatured with acetonitrile and the flocculant filtered out, allowing 96 samples to be processed in parallel.

Based on the industry standard MicroLute™ format, but without the chromatographic sorbent, protein ‘crashes’ out of solution and precipitates directly in each well when acetonitrile is added, thus solving all common problems associated with the CRASH technique of protein clean-up. The novel dual frit, hydrophobically treated matrix used by the Microlute™ PPP means that there is no ‘wetting out’ and leakage of sample before the application of vacuum.

Due to its ease of use, high performance and reliability the Microlute™ PPP is commonly used as the protein precipitation plate of choice in many major pharmaceutical companies worldwide.

To review the operational protocol for the Microlute™ PPP please visit To watch a video demonstration of the Microlute™ PPP please visit

For further information on the Microlute™ PPP please contact Porvair Sciences on +44-1978-666222 or [email protected].

Established in 1992, Porvair Sciences is one of the largest global manufacturers of ultra-clean microplates and top-quality microplate equipment for life science, synthetic chemistry and many other applications. Throughout North America – Porvair Sciences products are available and locally supported from sister company – JG Finneran Associates Inc. which also designs, manufactures and distributes a complimentary range of specialist laboratory consumables including vials, filters, microplates and closures. Porvair Sciences is a wholly owned subsidiary of Porvair plc.

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