Uncovering the Science Behind Thin-Layer Chromatography: How Does it Work? Kumar Jeetendra | October 30, 2023 Chemistry uses thin-layer chromatography (TLC) extensively as an analytical method for detecting and isolating chemicals from mixtures. It is founded on the ideas of chromatography, a technique for dissecting mixtures into their constituent parts. This is an explanation of TLC’s operation: The Chromatography Principle: Distribution of mixture components between two phases, a stationary phase and …