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    protein purification

    Using Ion Exchange Chromatography to Purify Proteins

    Proteins can be separated according to their net charge using the potent protein purification method known as ion exchange chromatography (IEC). Ion exchange chromatography can be used to demystify protein purification in the following ways: The Ion Exchange Chromatography Principle: The variations in net charge determine the protein separation of IEC. There are charged groups,