Everything You Need to Know About Ion exchange chromatography Kumar Jeetendra | March 18, 2023 The separation of ionizable molecules according to their aggregate charges is the goal of the chromatographic technique known as ion exchange chromatography. Because the charge carried by the molecule of interest can be easily changed by changing the buffer pH, this technique permits the separation of comparable types of molecules that would be difficult to …
Using Ion Exchange Chromatography to Purify Proteins Kumar Jeetendra | February 23, 2024 Proteins can be separated according to their net charge using the potent protein purification method known as ion exchange chromatography (IEC). Ion exchange chromatography can be used to demystify protein purification in the following ways: The Ion Exchange Chromatography Principle: The variations in net charge determine the protein separation of IEC. There are charged groups, …
