UPLC vs HPLC: Unraveling the Similarities and Differences Kumar Jeetendra | October 16, 2023 Both ultra-performance liquid chromatography (UPLC) and high-performance liquid chromatography (HPLC) are advanced analytical procedures used to separate and evaluate the constituent parts of complex mixtures. Despite their many similarities, they have important differences that serve to distinguish them. Here, we’ll unravel the similarities and differences between UPLC and HPLC: Similarities: Chromatographic Principle: The same basic …