Lab safety: Best Practices for Centrifuge Tube Usage and Storage

Lab safety: Best Practices for Centrifuge Tube Usage and Storage


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  • Source: Microbioz India

  • Date: 30 Nov,2023

It is crucial to ensure proper usage and storage of centrifuge tubes for laboratory safety.
Best Practices:

Centrifuge Tube Usage:

Check for Compatibility:

Make sure that the centrifuge tubes are compatible with the kind of centrifuge you have. Proper function in certain centrifuges requires specific tube types or adapters.

Inspect for Damage:

Before using, visually check each of the tubes if there are any cracks, chips or any other defect. This may lead to reduced integrity during centrifugation.

Proper Sealing:

To prevent leaks while conducting a centrifugation, you need to make sure that your tubes are sealed correctly. Use appropriate caps or closures and tighten them properly.

Balancing Loads:

Load the rotor evenly when operating the centrifuge in order to maintain balance. Imbalanced loads can cause vibrations and potential damage to the tube and rotors.

Also read:

Choosing the Right Centrifuge Tube for Your Lab Experiments

Maximum Speed and RCF:

Observe maximum speed in revolutions per minutes (rpm) as well as relative centrifugal force (RCF) limits specified for the particular centrifuge tubes used. Tube failure leads to unsafe flying away of things from their containment due to surpassing these limits.

Use Cushioning Material:

In such cases use cushioning materials or adapters recommended by the manufacturer to take extra care of them so that they do not break easily.

Temperature Considerations:

Be particularly aware of temperature limitations applicable on these tubes, especially when working with samples that require specific temperature conditions. Do not exceed beyond recommended temperature range.


Each tube should be clearly labeled with all relevant information including sample type, date, and any special handling instructions. This will enable a person to identify it and trace it back.

Centrifuge Tube Storage:

Clean and Dry:

Tubes should be thoroughly cleaned before being stored and allowed time to dry completely. Residual liquids or contaminants can interfere with subsequent experiments.

Organized Storage:

Store these tubes in an orderly manner to avoid confusion and prevent breakages or spillages.

Avoid Direct Sunlight:

Tubes should be kept away from direct sunlight and stored in cool dry places. This may compromise the tube integrity and stability of samples exposed to such conditions.

Use Rack Systems:

Use appropriate rack systems for storage to prevent tubes from tipping over. This reduces the risk of breakage and spillage.

Compatible Storage Conditions:

Beforehand, know the specific storage conditions prescribed for these tubes such as temperature, humidity or light blocking properties. Comply with the manufacturer specifications.

Regular Inspection:

Check stored tubes periodically for any signs of damage or degradation. Throw away those that show visible wear, cracks or other issues.

Segregate Hazardous Materials:

It is important when storing hazardous materials to segregate them properly and mark containers with hazard information. I stock hazardous substances according to safety guidelines.

Doing so ensures that your samples are safe while working with centrifuge tubes thus minimizing chances of accidents and a stable laboratory environment.

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