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Spectrophotometry is a method for determining the amount of light a chemical substance absorbs by measuring the intensity of light as a light beam passes through a sample solution. The fundamental idea is that each substance absorbs or transmits light within a specific wavelength range. This measurement can also be used to determine the concentration of a known chemical compound. In domains such as chemistry, physics, biochemistry, material and chemical engineering, and therapeutic applications, Spectrophotometry is one of the most valuable quantitative analysis techniques.
A Spectrophotometer is an equipment that measures the amount of photons (light intensity) absorbed by a sample solution after light has passed through it. By measuring the observed intensity of light with a spectrophotometer, it is also possible to measure the concentration of a given chemical compound.
Utilizes Electromagnetic Energy within the ultraviolet (185 – 400 nm) and visible (400 – 700 nm) regions of the spectrum.
Makes use of light in the infrared region of the Electromagnetic Radiation Spectrum, which extends from 700 to 15000 nanometers.
The study of the relationship between radiated matter and emitted energy is known as spectroscopy. After taking in the energy, the material goes into an excited state, which produces an easily observable interaction through the visible light produced by electromagnetic waves on the visible spectrum. In more recent times, this field of study has broadened to incorporate the interactions that take place between protons, ions, and electrons. In the scientific disciplines of physics, chemistry, and astronomy—all of which have advanced as a direct result of the findings of this study—spectroscopy is a technique that is utilised frequently.
In spite of the fact that the terms spectrophotometry and Spectroscopy are often used interchangeably; these two disciplines are quite distinct from one another in many respects:
Spectrophotometer: Representative image