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Ultraviolet-visible (UV-Vis) spectroscopy is a widely used analytical technique trusted in many areas of science including chemistry, physics, biochemistry, chemical engineering, and clinical applications.
UV-Visible Spectroscopy is based on the absorption of ultraviolet light or visible light by chemical compounds, often with chromophoric functional groups, which results in the production of distinct spectra. Spectroscopic techniques are based on the interaction between light and matter. When the matter absorbs the light, it undergoes excitation and de-excitation, resulting in the production of a spectrum.
When matter absorbs ultraviolet radiation, the electrons present in it undergo excitation. This causes them to jump from a ground state to an excited state. It is important to note that the difference in the energies of the ground state and the excited state of the electron is always equal to the amount of ultraviolet radiation or visible radiation absorbed by it. This short article describes an inline UV-Visible spectrophotometer optimised for real time monitoring of continuous flow and batch applications.
UV-Visible spectrophotometer
The compact Flow-UV UV-Visible spectrophotometer from Uniqsis was purpose designed for real time monitoring of continuous flow and batch applications. Utilising a Xe strobe lamp source with a lifetime of up to 10 years in normal use, coupled with a CCD array, unlike many traditional UV-visible spectrophotometers the Flow-UV requires no routine maintenance or calibration and can record full spectra in the range 190-1050nm.
The Flow-UV uses fibre optic waveguides that connect the flow cell to the detector, allowing the flow cell to be positioned virtually anywhere in the flow path. To assure linearity of response, up to 5 wavelengths can be selected to monitor the progress of a reaction or monitor concentration changes in a wide variety of media. The Flow UV is supplied with 2 software applications for both flow (dynamic) and batch processes.
Because of these inherent advantages, demand for the Flow-UV in biological, biotech, drug discovery and industrial applications has grown to record levels.
Commercial Director for Uniqsis commented “During the last 12 months, one biotech customer ordered 6 Flow-UV units for nucleic acid purity assessment. We have also seen repeatedly in pharma labs that the unit is being used for high throughput screening of UV-active drug substances and real time determination of the steady state concentration of products being produced in drug discovery reaction optimisation studies”.
He added “Customers really appreciate the intuitive method set up and simple data acquisition interface.
As a consequence, researchers are also using the Flow-UV for monitoring algal growth, looking at variations of different metal ion complex solutions in rock cores and optimising microporous membrane production to name but a few of the wide array of applications this versatile spectrophotometer was selected for”.
For further information about the Flow-UV UV-Visible spectrophotometer please visit www.uniqsis.com/paProductsDetail.aspx?ID=Flow%20UV%20ACC or contact Steve Evans of Uniqsis on +44-845-864-7747 / steve.evans@uniqsis.com.
