Overview

• Post By : Kumar Jeetendra


• Source: Microbioz India


• Date: 02 May,2025

Whether you are in a pharmaceutical lab, doing biological research, or in clinical diagnostics, pipetting is one of the tasks which captures your attention in one way or another. of your experience, all professionals encounter issues such as air bubbles, inconsistent volumes, and hand fatigue.

Every trained professional knows exactly how important it is to irritate the tip of the pipette with a specific pre-wasting technique.

Here are some handy tips to help you succeed on your next pre-warming session.

Prior to Pre-Displacing the Sample

A decidedly helpful recommendation for improving the tip pre-waste is to aspirate and dispense the same fluid once or twice before drawing the actual sample.

Right the Job Right Pipette and Tip Number Two

1. Every tip and pipette a professional use must go along more advanced caps, otherwise pre-wasting and utter wet interface wasting will not occur.
2. Observe Set Degrees Or more commonly known as an ergonomic approach, aspire negative tilt with your signature lifted about 60 degrees, giving the perfect horizontal view.
3. Hover Tips in the Liquid at the Right Height
4. You can make a mistake if you go too deep or too shallow into the liquid. You want to place the tip just beneath the surface, somewhere around 2–3 mm for smaller volumes and 3–6 mm for larger ones.

Sip in and Sip Out Smoothly and Continuously

Abrupt and hurried slips of the thumb have no place here. Aspirate and dispense with a constant pace. Fast aspiration tends to trap air bubbles within, and aggressive dispensing causes splashing or foamer. In addition, only serve smooth and consistent splaying.

Hold After Drawing Liquid

After drawing up the aliquot, hold the pipette upright for 1-2 seconds before removing the tip from the sample. This ensures that the full volume is guaranteed to be within the tip, therefore minimizing variation.

Avoid Air Bubbles with Everything You Have

Always look at the tip while aspirating – air bubbles are universally agreed to be a result of poor technique. Air bubbles within the liquid in conjunction with any surrounding liquid lead to inaccurate measurements of sample volume and sample contamination. All bubbles render invalid results; thus samples need to be discarded.

Maintain Accurate and Clean Pipettes

Miscalibrated pipettes will hardly ever produce accurate results. These mechanical workhorses require thorough maintenance: clean the piston and shaft every so often, then seek professional calibration every 3-6 months according to usage.

Switch to Electronic Pipettes to Ease Repetitive Work

For activities like ELISA or qPCR where volume repetition is necessary, it might be beneficial to invest in electronic or multi-channel pipettes. They enhance both speed and consistency and lessen hand and wrist strain.

Maintain Room Temperature Conditions

Rooms should be temperature-controlled with pipettes and liquids kept at room temperature. Refrigeration may cause liquids to expand, which could distort the true volume. If refrigeration is used, samples should be allowed to warm for 15–30 minutes.

The Bottom Line

Pipetting is a task that involves fine motor skills. With the right practices, it is possible to reduce errors and increase reliability without adding extra load on hands or experiments.