Microbioz India: 9 tips to Keep in Mind When Purchasing HPLC Columns

Microbioz India: 9 tips to Keep in Mind When Purchasing HPLC Columns


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  • Source: Microbioz India

  • Date: 14 Jul,2023

Differences in size the majority of HPLC columns are used for protein and carbohydrate separations. Racemic mixture components that cannot be separated using conventional HPLC column types, such as affinity, ion exclusion, or displacement chromatography, can be separated using chiral HPLC columns.

Sure! Here are 9 tips for buying HPLC columns:

  1. Analytical requirements: Specify your analytical requirements, including sample type, analytes of interest, separation objectives, and detection limits. This information will assist you in selecting the column that best meets your requirements.
  2. Consider the column’s dimensions, including its length, inner diameter, and particle size. These variables can have a substantial effect on the separation efficacy and analysis time. Select the column dimensions that correspond most closely with your analytical objectives and instrument capabilities.
  3. Choose a stationary phase chemistry that is compatible with the matrix and analytes of your sample. Reversed-phase, normal-phase, ion-exchange, and size-exclusion are widespread stationary phase chemistries. If you are uncertain about the best option, consult the relevant literature or consult an expert.
  4. Purchase columns from manufacturers with a solid reputation for producing high-quality, consistent products. Typically, established manufacturers have superior quality control and offer dependable technical support.
  5. Compatibility with the sample: Ensure the column is compatible with the sample’s solvent, mobile phase, and pH conditions. Some samples may require columns designed specifically for severe or extreme environments.
  6. Column performance and lifetime: Review the manufacturer-supplied column performance specifications, such as peak capacity, efficiency, and lifetime. Consider the column’s anticipated lifespan when making a purchase decision to maximize cost-effectiveness.
  7. Determine your budget and search for columns that provide a decent balance between quality and price. Although price is essential, do not sacrifice quality, as cheaper columns may produce inferior results or have shorter lifetimes.
  8. Check user reviews and ask seasoned chromatographers for advice in order to determine the column’s reputation. Other users’ feedback can provide valuable insight into column efficacy, reproducibility, and durability.
  9. Column compatibility: Ensure that the column hardware (e.g., dimensions, connectors, and connections) is compatible with your HPLC system. Check the manufacturer’s specifications for both the column and the instrument to ensure compatibility.

Consider the availability and caliber of the technical support provided by the column manufacturer. Support that is prompt and knowledgeable can be crucial for resolving issues and optimizing column performance.

HPLC Columns: Frequently Asked Questions (FAQs)

What is a Chiral HPLC column ?:

Chiral chromatography is the process of separating enantiomers by utilizing a chiral HPLC column. A chiral HPLC column is an HPLC column that is packed with a chiral stationary phase (CSP). Chiral chromatography is also known as chiral chromatography. Enantiomers are separated depending on the number of interactions that take place during the time that they are exposed to the chiral stationary phase as well as the nature of those interactions.

What is C18 column HPLC ? :

C18 column high performance liquid chromatography: C18 columns are a type of HPLC (high performance liquid chromatography) column that use a C18 material as the stationary phase. In the fields of environmental research and chemical analysis, as well as in sectors such as pharmaceutical and environmental sciences, C18 HPLC columns are utilized in order to examine specific components of chemical mixtures.

Here are few brands offering HPLC columns:

Waters HPLC columns:

Waters provides customers with a diverse selection of column options and formats, including as HPLC, UPLC, and UHPLC, as well as GPC, SFC, and SEC columns. These columns are all constructed using dependable quality manufacturing, which ensures that procedures created today will have the same reproducible results tomorrow. 

Agilent HPLC columns:

Agilent is responsible for the entire design and production process, from beginning to end, of reversed-phase high-performance liquid chromatography (HPLC) columns. During the manufacturing process of the column, several Quality Control (QC) tests are performed. These tests ensure that the column will have a robust performance that is also reproducible, as well as a long lifetime. The InfinityLab Poroshell 120, Zorbax, Pursuit, XRs and XRs Ultra, Polaris, and HC-C18(2) and TC-C18(2) are all examples of columns that can be used in reversed-phase HPLC.

 If you want dependable chromatographic findings, remember that picking the correct HPLC column is crucial. Use these suggestions as a starting point, and modify them to fit your needs and interests.

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