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Fetal Bovine Serum (FBS) holds great value in cell culture and is applied in practically all biomedical research as well as in pharmaceuticals and biotechnology. FBS, being a major source of nutrients for cells, ensures the best conditions for cell growth and maintenance. Choosing the right FBS can determine the success of an experiment.
sFBS in a contamination environment will have reduced FBS and more impurities compared to FBS not affected by contamination. It is recommended that clients look for products that underwent quality and quantity restraints filtration alongside reliability consistent tests.
FBS based on different cultures can differ in performance. After contracted FBS is tested, purchasing bulk amounts can be done. It is wise to ensure that the test FBS has appropriate cell lines and proper experimental conditions to maximize achievement.
The geographical origin of FBS can impact its composition. Also important is the ethical procurement of the FBS. Check that the animal products utilized do not violate any animal welfare policies or industry regulations
FBS with complement proteins that may inhibit the growth of cells need to be heat treated to deactivate them. While using serum for the experiments, understand whether heat will be beneficial or not for your research.
In order for FBS to remain stable and effective, it is critical to store it a -20 °C or cooler. Avoid multiple freeze-thaw cycles, and thaw FBS slowly to make sure it does not deteriorate.
Forecasting costs is essential, but purchasing the least expensive option often proves to be counterproductive. Evaluate reputation, batch-to-batch variability, and even the level of technical assistance offered by the provider.
Quality Fetal Bovine Serum is still the most optimal approach in achieving successful outcomes in cell culture. Enhanced precision and consistency of your experiments is ensured with proper storage conditions and FBS optimization.
