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DPX Technologies, Columbia, SC, has launched a new ultra-pure tier filtration tip Together with a new program note with Hamilton Robotics, Reno, Nev, titled”Automated Method to get High-Throughput LC-MS/MS Quantitation of Testosterone from Serum: An Improved Validated Method.”
The method for the extraction of testosterone in serum used Low Porosity Filtration Tips-Ultra Pure (LPFT-UP) and incorporated a ZnSO4solution in addition to acetonitrile on a Hamilton Heater Shaker (HHS) for enhanced recovery and precision through protein precipitation. The LPFT-UP offer a high quality filtration media for methods with very low detection limits.
Patient samples had a calculated recovery of testosterone at 99.5% without the detected matrix effects. This shows the use of ZnSO4and acetonitrile is quite effective in extracting testosterone, and also the usage of LPFT-UP resulted in negligible losses. The debut of Low Porosity Filtration tips-Ultra Pure supplies a brand new product for non detection level procedures.
“We are excited to expand our product offering for steroid testing,” says Carmen Adamson, brand director. DPX plans to expand on the research and development to include a full panel of steroids.
“Testosterone imbalance can signal a problem in both women and men. This method gives an accurate and precise tool to aid in that decision,” states Evan DiVirgilio, application scientist.
For more information, visit DPX Technologies.
