Mastering the Art of Blotting: Essential Techniques for Beginners Kumar Jeetendra | November 22, 2023 Various molecular biology applications require skillful blotting techniques mastering. Here are the basic techniques for beginners: Southern Blotting (DNA): Purpose: To identify specific DNA sequences Steps: DNA Digestion: DNA cut by restriction enzymes. Gel Electrophoresis: Separation of DNA fragments by size using an agarose gel. DNA Denaturation: Alkaline treatment of the DNA to denature it. …
