Affinity Chromatography

Affinity chromatography, a method used to separate complex mixtures of proteins on the basis of their specific biological affinity for ligands immobilized on a solid support, is one such technique. Techniques of Affinity chromatography: Ligand selection: Affinity Ligand: Selecting a ligand with high specificity for the target molecule Immobilization: Method of coupling the ligand to chromatographic …